8 Module 8: Share Your Results
8.1 Background
Write a short paragraph describing your strain, the experiment your team will perform, the science behind it, and how the experiment supports your final research goal.
Add background here.
8.2 Criteria for Success
Successful completion requires full participation in the designed experiment, careful documentation, and a complete ELN entry with results and reflection.
8.3 Learning Outcomes
- List safety considerations for working with microbial isolates. (CO 3, 4, 5) 🥼
- Review techniques, concepts, and experiments from the course. (CO 3, 4, 5) 🥼
- Practice repeating experiments to improve consistency and reproducibility. (CO 3, 4, 5) 🥼
- Describe the purpose, methods, and results of the experiment your team designs. (CO 2, 3, 8,9) 📁
- Interpret genomic data, including gene annotations and protein interaction predictions. (CO 6, 7, 8) 📈
- Revise the draft for your individual and group projects. (CO 3, 9) 🏅
- Analyze how this experiment contributes to your overall experimental goal. (CO 1) 🤔
8.4 Skills and Knowledge
Completing this module will help you develop and practice the following skills and knowledge, which you can include in your curriculum vitae.
Skills: This module helps you practice the following essential skills for research and experimental preparation and execution. After completing this lab, you will gain the following lab skills:
Add Skills here.
Knowledge: This module will also help you become familiar with the following important content in microbiology lab settings:
Add knowledge here.
8.5 Procedures
8.5.1 Lab Safety
8.5.1.1 Before Lab
- Wear the correct PPE (lab coat, goggles, and gloves).
- Wipe the lab bench with 70% ethanol.
- Before working with microorganisms, carefully wipe pipettors with 70% ethanol.
8.5.1.2 During Lab
- Treat all tips as potential biohazards.
- Remove your PPE if you are leaving the lab (water/bio break).
- Discard samples and disposable lab items in the correct trash bins.
- Try to keep personal items (computers, phones, etc.) usage to a minimum.
8.5.1.3 After Lab
- Wipe your station with 70% alcohol.
- If you touched any personal items during the lab, wipe them with 70% ethanol before leaving.
- Dispose of gloves in the main biohazard waste container.
- Dispose of used petri dishes in the biohazard bin.
- All liquids containing bacteria must be poured into a beaker for bleach decontamination (can be found on the blue bench paper by this sink labeled “liquid”).
- Glass tubes can be placed into a beaker (on the blue bench paper by this sink, labeled “glass”).
- All bacterial growth plates can be thrown away in the biohazard trash.
- Return all reusable materials to our storage drawers.
- Wipe down all surfaces with ethanol.
- Remove PPE.
- Wash your hands.
8.5.2 Methods
Write your Methods/Procedures here. The purpose is to enable someone else to replicate your experiment, giving as much detail as needed. Include your data analysis plan as well.
Replace the note with the description of the methods you used.
8.6 Data/Results
What results did you get, and what do they mean? Share your results!
Replace the note with your results, add images if applicable.
8.6.1 Result Analysis
Write an analysis of your results; explain what these results mean, and whether this matches what was expected
Replace the note with your result analysis.
8.7 Discussion
- What did you learn from exploring your genome assembly and annotation with BV-BRC and SeqHub?
- Were you able to conduct and repeat your proposed experiment at least once? Why or why not?
- What findings did you obtain, and what are their implications?
- What experiment would you do next, and why?
Replace the note with your discussion.